mirror of https://github.com/CrimsonTome/tldr.git
30 lines
1.3 KiB
Markdown
30 lines
1.3 KiB
Markdown
# bedtools
|
|
|
|
> A swiss-army knife of tools for genomic-analysis tasks.
|
|
> Used to intersect, group, convert and count data in BAM, BED, GFF/GTF, VCF format.
|
|
> More information: <https://bedtools.readthedocs.io/en/latest/>.
|
|
|
|
- Intersect two files regarding the sequences' strand and save the result to the specified file:
|
|
|
|
`bedtools intersect -a {{path/to/file_1}} -b {{path/to/file_2}} -s > {{path/to/output_file}}`
|
|
|
|
- Intersect two files with a left outer join, i.e. report each feature from {{file_1}} and NULL if no overlap with {{file_2}}:
|
|
|
|
`bedtools intersect -a {{path/to/file_1}} -b {{path/to/file_2}} -lof > {{path/to/output_file}}`
|
|
|
|
- Using more efficient algorithm to intersect two pre-sorted files:
|
|
|
|
`bedtools intersect -a {{path/to/file_1}} -b {{path/to/file_2}} -sorted > {{path/to/output_file}}`
|
|
|
|
- Group file {{`path/to/file`}} based on the first three and the fifth column and summarize the sixth column by summing it up:
|
|
|
|
`bedtools groupby -i {{path/to/file}} -c 1-3,5 -g 6 -o sum`
|
|
|
|
- Convert bam-formatted file to a bed-formatted one:
|
|
|
|
`bedtools bamtobed -i {{path/to/file}}.bam > {{path/to/file}}.bed`
|
|
|
|
- Find for all features in {{file_1}}.bed the closest one in {{file_2}}.bed and write their distance in an extra column (input files must be sorted):
|
|
|
|
`bedtools closest -a {{path/to/file_1}}.bed -b {{path/to/file_2}}.bed -d`
|